Coding
Bglx

Part:BBa_K4211018:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-19)


Bglx: β-glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region.
  • Constructed by PCR.
  • We added a 6xHis tag at the end of the gene to purify the protein. To terminate transcription, we added the TAA sequence.

Forward primer

5'- GTG GGC GAC CGA TTC CAG -3'

Reverse primer

5'- TAT TAA TAA CTA TTA GTG ATG GTG ATG GTG ATG GGG CTG GTA GGT CGC G -3'

Source

The source of this section is a sequence retrieved from GenBank.

References

Méndez-Líter, J. A. et al. A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds. Microb Cell Fact 19, 127 (2020). https://doi.org:10.1186/s12934-020-01386-1